How to remove vitreous?

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Abhinaba
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How to remove vitreous?

I want to record  (patch lamp, whole cell)  from  retinal ganglion cells and bipolar cells  (mouse model).  But I am unable to remove the viterous  and  inner limiting membrane,  even  after  30 min treatment  with  hyaluronidase  (0.5 mg/ml) !!  Can somebody please help!!