Need help identifying cortical regions in CO-stained horizontal sections

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ecolechio
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Need help identifying cortical regions in CO-stained horizontal sections

I have been making retrograde tracer injections into rat motor cortex, flattening the cortical hemispheres, and sectioning them tangentially/horizontally with CO processing through layer IV sections. I do not want to analyze the labeling in MI cortex ipsilateral to the injection site, but I would like to include the cingulate and retrosplenial cortices in my data analysis since previous studies have shown that cells in these regions do project to MI. The problem is that I am having trouble differentiating between MI cortex (which is obviously filled with very dense labeling) and these other regions. At this point, I am willing to pool the data from cingulate and retrosplenial cortices, but I still need to be able to decide where to draw the boundary for MI cortex. Any ideas?

Arvind Singh Pundir
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i will let u know with the

i will let u know with the help of my friends within a day

Fraser Moss
Fraser Moss's picture
This field is not my

This field is not my specialty, but perhaps some information in this paper may help you?

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2504743

J Comp Neurol. 2008 September 1; 510(1): 100–116.
Contralateral Corticothalamic Projections from MI Whisker Cortex: Potential Route for Modulating Hemispheric Interactions
Kevin D. Alloway,* Michelle L. Olson, and Jared B. Smith

PMID: 18615539

http://www.ncbi.nlm.nih.gov/sites/entrez?orig_db=PubMed&db=pubmed&cmd=Search&term=510[volume]%20AND%20100[page]%20AND%202008[pdat]

ecolechio
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Haha! Thanks. They are using

Haha! Thanks. They are using coronal sections, unfortunately. I need to figure this out in tangential sections - that's my problem. :/

ddlarsen
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Try using an additional stain

Try using an additional stain such as a myelin stain. It will let you see a few more areas in the tangential sections.

ecolechio
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Thanks

Thanks

Neuron_irranna
Neuron_irranna's picture
Hey!

Hey!
If you have done CO staining on the flattened cortex sections of rat, you should be able to visualize the barrel cortex. Now to dilineate the motor cortex, you can refer to paper which was published from my lab recently on the normal organization of rat motor cortex (Overlapping representations of the neck and whiskers in the rat motor cortex revealed by mapping at different anaesthetic depths. Shashank Tandon, Niranjan Kambi and Neeraj Jain. European Journal of Neuroscience, pp. 1–10, 2007). In the paper we have shown the extent of motor cortex with respect to the barrel cortex on flattened cortex. Using this you can mark the motor cortex in your rat sections. I hope this info helps. I have attached the paper for your reference.

ecolechio
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Thank you!! This is very

Thank you!! This is very interesting as we have been doing tons of ICMS as well. Thanks so much!

Neuron_irranna
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Here is the paper...

Here is the paper...

ecolechio
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Thanks again. :)

Thanks again. :)