How to analyze the results from ramp protocol by patch clamp

1 post / 0 new
keyong
keyong's picture
How to analyze the results from ramp protocol by patch clamp

Hi, Say hello to all of you. It is my first time to come here. Waa, it is great!

Now I am trying to studying inwardly rectifying potassium channels in chemosensitive neurons. I read one paper titled with "carbon dioxide regulates the tonic activity of locus coeruleus neurons by modulating a proton- and polyamine-sensitive inward rectifier potassium current". I have a question which is how to analyze the traces by subtraction of basal trace from experimental trace? What is the positive or negative slope conductance means ?

Thank you very much!

Keyong