BDA injection

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rjgeevaratne
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BDA injection

Does anyone know if BDA will travel more than one synapse? If so, what is the time-frame and will the label at the second synapse be as robust as that at the first?

heping zhang
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rjgeevaratne wrote:Does

rjgeevaratne wrote:

Does anyone know if BDA will travel more than one synapse? If so, what is the time-frame and will the label at the second synapse be as robust as that at the first?

What is BDA please?

Soudabeh
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heping zhang wrote

heping zhang wrote:

rjgeevaratne wrote:
Does anyone know if BDA will travel more than one synapse? If so, what is the time-frame and will the label at the second synapse be as robust as that at the first?

What is BDA please?

BDA stands for Biotinalated Dextran Amine, it is used in form of injections to study, by electron microscopy , the neuron's function at the synapses area.

jongi1
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rjgeevaratne wrote:Does

rjgeevaratne wrote:

Does anyone know if BDA will travel more than one synapse? If so, what is the time-frame and will the label at the second synapse be as robust as that at the first?

As far as I know, the dextran amines do not cross the synapse. Additionally, these tracers are taken up by other cells and therefore can be messy. You would have to time the tracing well otherwise these can leak and your histology would look bad.
I think WGA crosses the synapse so you might want to try it instead...also, it is transported faster than the dextran amines (...but maybe transport rate has something to do with the molecular weight of the dextran amine you are using).
Other transynaptic tracers include the pseudorabies virus and cholera toxin.
Good luck!

rjgeevaratne
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Thank you for your response,

Thank you for your response, I know that PRV is a good transsynaptic tracer, and am very familiar with Cholrea Toxin. I am hoping to use BDA to go one synapse, however the problem is that the brain area I will be injecting into is VERY close to the area I want it to trace to, so I was hoping to be able to go back a synapse, to the ganglia. Cholera Toxin is not a good choice because although it can be transported farther than one synapse, the amount of transported toxin gets exponentially smaller with each synapse. I am afraid I would lose the robustness of my label.

That said, is the size of BDA is the problem, has anyone used BDA smaller than 10,000 mw?

SciUser
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My lab has use it at 3K MW

My lab has use it at 3K MW and it has been working fine.

Aicanor
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I am thinking also of using

I am thinking also of using BDA to mark injection site in a peripheral nerve.

I will use iontophoretic injection of either 3k Da or 10k Da.
My question is - do you have data on its electrophoretic motility?

Suggestions for other tracers are welcome:
They should comply to the following requirements:
- The spot should endure fixation and long storage 4-6 weeks
- The spot should be laid off after relatively small electrophoretic time, i.e. small charge injection - safety limits

FarFletched
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Aicanor wrote:I am thinking

Aicanor wrote:

I am thinking also of using BDA to mark injection site in a peripheral nerve.

I will use iontophoretic injection of either 3k Da or 10k Da.
My question is - do you have data on its electrophoretic motility?

Suggestions for other tracers are welcome:
They should comply to the following requirements:
- The spot should endure fixation and long storage 4-6 weeks
- The spot should be laid off after relatively small electrophoretic time, i.e. small charge injection - safety limits

Try using Molecular Probes DiI or DiO paste or crystals, or even a tiny piece of charcoal.