Title: Assistant Specialist Step I- Step III-Neuroscience - Molecular and Cell Biology Assistant Specialist: We are seeking a highly motivated individual to support the Ngai lab’s BRAIN Initiative project to demonstrate feasibility on applying several new technologies, including high-throughput single cell transcriptome profiling and CRISPR-based genome engineering, to the characterization of cellular diversity in the mammalian cerebral cortex. As part of an NIH-funded BRAIN Initiative project that includes 10 research groups and 3 technology core facilities on the UC Berkeley campus, this individual will be an integral member of a dynamic, multidisciplinary team of neuroscientists, molecular biologists, genomics technologists, mouse geneticists, statisticians and computer scientists to develop and deploy new technologies for creating a taxonomy of cell types in the brain. Responsibilities: The successful candidate will assist and collaborate with senior lab personnel on the following aspects of the Ngai Lab’s NIH-funded BRAIN Initiative project: preparation of cells and reagents for single cell RNA sequencing experiments; processing and quality control of samples for high-throughput Illumina sequencing; single- and double-label RNA in situ hybridization; design and preparation of DNA and RNA constructs for CRISPR-based genome engineering of mouse ES cells and mouse zygotes; ES cell culture and genome engineering; organization and archiving of data for reports and publication.Minimum/Basic Qualifications Required (By the time of application):Applicants must possess a bachelor’s degree or equivalent degree in biology or related field.Additional Required Qualifications (By start date):Hands-on experience in routine molecular biology (molecular cloning, PCR, plasmid design and construction; cell culture including ES cell culture and transfection; microfluidics technologies; biochemistry; immunohistochemistry; single- and double-label RNA in situ hybridization; handling samples in and organizing data from high-throughput format experiments. Hands-on experience with epifluorescence and confocal light microscopy.Preferred Qualifications (By start date):Working knowledge in mammalian neuroanatomy and bioinformatics analysis; experience working in a research laboratory.Appointment:This position reports to Dr. John Ngai. The initial appointment will be for 100% time for 1 year with the expectation of extension based on availability of funds. The approximate start date for this position will be May 2016.Salary: $42,780.00 - $48,768.00 depending on qualifications. This position provides full benefits.To Apply: Visit https://aprecruit.berkeley.edu/apply/JPF00976Interested individuals should submit application documents as PDFs, which includes, an updated curriculum vitae, and names with contact information for at least 3-5 individuals who have agreed to provide a reference for this specific position and a cover letter. (Letters of reference may be requested of the finalists).Department Contact: Terri Leong, HR Partner. firstname.lastname@example.orgThis recruitment will remain open until filled.All letters will be treated as confidential per University of California policy and California state law. Please refer potential referees, including when letters are provided via a third party (i.e., dossier service or career center), to the UC Berkeley statement of confidentiality (http://apo.berkeley.edu/evalltr.html) prior to submitting their letters.The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see: http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct.The department is interested in candidates who will contribute to diversity and equal opportunity in higher education through their work.