re-sealing in patch clamp

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asterix
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re-sealing in patch clamp

We are having problems with our patch electrodes resealing after 2 or 3 minutes. the resistance is great ( 2.5-3), we obtain Gigaseals ina more or less fast, the series resistance is very good (~7) and then the electrode re-seal, and we battle for a while breaking the seal, but it reseal again and finally we kill the cell. Any advice?

chrismathes
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 It sounds like you have a

 It sounds like you have a pressure leak. Check the o-ring in your holder. 

asterix
asterix's picture
 Thanks!

 Thanks!