http://www.nottingham.ac.uk/pathology/protocols/pasog.html
1. Sections to water.
2. Treat with 1% periodic acid for 10 minutes.
3. Wash in water.
4. Stain in Schiffs reagent for 10 minutes.
5. Wash in tap water until sections appear pink.
6. Treat with 4% iron alum for 5 minutes.
7. Wash in distilled water.
8. Stain nuclei with Mayers haematoxylin for 5.minutes.
9. Differentiate in 1% acid alcohol.
10. Blue in tap water.
11. Stain with 0.2% orange g in alcoholic picric acid.
12. Dehydrate in absolute alcohol and examine microscopically.
13. Differentiate in 70% alcohol until the background appears colourless.
14. Dehydrate, clear . Mount sections in DPX
RESULTS
--Basophil cells magenta
--Acidophil cells yellow
--Red blood cells yellow
--Nuclei blue/black
--Chromophobes pale blue/grey
SOLUTIONS
Schiffs reagent
Can I ask why the 4% iron alum step
I think 4% Alum acts as a mordant for the haematoxylin used in the next step ...............