PERIODIC ACID SCHIFFS ORANGE G

http://www.nottingham.ac.uk/pathology/protocols/pasog.html

1. Sections to water.

2. Treat with 1% periodic acid for 10 minutes.

3. Wash in water.

4. Stain in Schiffs reagent for 10 minutes.

5. Wash in tap water until sections appear pink.

6. Treat with 4% iron alum for 5 minutes.

7. Wash in distilled water.

8. Stain nuclei with Mayers haematoxylin for 5.minutes.

9. Differentiate in 1% acid alcohol.

10. Blue in tap water.

11. Stain with 0.2% orange g in alcoholic picric acid.

12. Dehydrate in absolute alcohol and examine microscopically.

13. Differentiate in 70% alcohol until the background appears colourless.

14. Dehydrate, clear . Mount sections in DPX

RESULTS

--Basophil cells magenta

--Acidophil cells yellow

--Red blood cells yellow

--Nuclei blue/black

--Chromophobes pale blue/grey

SOLUTIONS

Schiffs reagent