Neuron culture protocol

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vdas
vdas's picture
Neuron culture protocol

Hi,

Can anyone let me know a good protocol for sympathetic neuron culture from rats?

thanks

The FFM
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It's old but the following

It's old but the following paper has a protocol for culture of sympathetic neurons from both embryonic and postnatal rats

http://jn.physiology.org/cgi/reprint/42/5/1410

http://www.ncbi.nlm.nih.gov/pubmed/39983

another paper describing SCG preparation is http://www.ncbi.nlm.nih.gov/pubmed/3601217

and a much more up to date video protocol for culturing sympathetic neurons from rat superior cervical ganglia (SCG) can be found here http://www.jove.com/index/details.stp?id=988