Has anyone ever measured resting potentials in primary neurons grown in Neurobasal-based cell culture media and compared to neurons grown in DMEM?
I grow E18 rat dissociated hippocampal cultures and find that neurons in DMEM-based media are much more exciteable and have much higher levels of spontaneous activity than when grown in neurobasal. Anyone else had a similar experience?
I noticed in the recipes, that the Na+ concentration in neurobasal is only about 58 mMol, versus about 120 for DMEM. Shouldn't make too much difference in resting potential, but maybe just enough. Also, maybe development if effected by abnormally low concentrations, like those that are found in neurobasal?