EAATs antibodies NEED HELP!

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luciotremolizzo
luciotremolizzo's picture
EAATs antibodies NEED HELP!

I have tried to work out the western blotting assays for EAAT-1, -2 and -3 using Chemicon Abs but they did not work very well... now it even seems to me that any previously published gel is not REALLY nice.
Is actually somebody able to get a nice immunoreactivity for EAATs?
Is there any specific problem that I must address?
Thanks.

Fraser Moss
Fraser Moss's picture
Are you staining for Mouse,

Are you staining for Mouse, Rat or human EAATs?

Have you asked Jeff D Rothstein for samples of any of his antibodies (Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA)?

Search Pubmed for Rothstein JD to get many papers with EAAT immunoprobing.

luciotremolizzo
luciotremolizzo's picture
human... I tried the

human... I tried the Rothstein Ab previously but I was wondering if there are any commercially available ab that works ok.
thanks in any case

frasermoss wrote:

Are you staining for Mouse, Rat or human EAATs?

Have you asked Jeff D Rothstein for samples of any of his antibodies (Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA)?

Search Pubmed for Rothstein JD to get many papers with EAAT immunoprobing.

Fraser Moss
Fraser Moss's picture
I cannot vouch for its

I cannot vouch for its quality, but I found this Mouse Anti-Human Excitatory Amino Acid Transporter 2 Ab-1 Monoclonal Antibody, Unconjugated, Clone 1H8 from Lab Vision

http://www.labvision.com/ab.cfm?first=AntiBody&second=1750

Search biocompare.com for antibody suppliers

taurine
taurine's picture
Suggest look at the papers by

Suggest look at the papers by Pow DV who has some nice antibodies to all the EAATs including splice variants. There is a company (immunosolution.com) that sells some of them (I think)

taurine
taurine's picture
PS see the EAATs website at

PS see the EAATs website at www.eaats.org as it has some nice images of EAATs immunocytochemistry including human.

luciotremolizzo
luciotremolizzo's picture
Thanks a lot... I did not try

Thanks a lot... I did not try these Abs before... I'll let you know if I buy them. L