CRESYL FAST VIOLET TECHNIQUE FOR NISSL

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Tracy
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CRESYL FAST VIOLET TECHNIQUE FOR NISSL

http://www.nottingham.ac.uk/pathology/protocols/cfvnissl.html

SECTIONS

8m paraffin wax sections

SOLUTIONS

STAINING SOLUTION

Cresyl Fast Violet-1g
Distilled water-100cm3
Acetic acid-0.25cm3

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METHOD

Take sections to water
Stain in cresyl fast violet solution for 20-30 minutes
Rinse in distilled water
Leave in 96% alcohol until most of the stain has been removed
Clear in xylene. Mount sections in DPX

RESULTS

Nissl substance-Purple/dark blue
Neurones and cell nuclei - Purple/blue