BIELSCHOWSKY METHOD

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Tracy
Tracy's picture
BIELSCHOWSKY METHOD

SECTIONS
8micron paraffin wax sections.

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SOLUTIONS

1. Silver A - 20% silver nitrate

2. Reducer (Make fresh)

Formaldehyde 50ml

Absolute Alcohol 200ml

Distilled water 750ml

3. Silver B

20% Silver nitrate 20ml

95% Alcohol 20ml

Add ammonia until precipitate formed is redissolved

Add 10 more drops of ammonia

4. 5% Sodium thiosulphate

5. 0.1% Gold chloride

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METHOD

Take sections to distilled water

Place in solution A for 2-3 hours

Wash in reducer for 2-5 minutes

Wash in 10% alcohol for 2 minutes

Place in solution B for 1-2 minutes

Wash in a constant flow of reducer and leave for 3-5 minutes

Wash in distilled water

Tone 0.1% gold chloride for 2 minutes

Wash in distilled water

Fix in 5% sodium thiosulphate for 2 minutes

Wash in tap water

Dehydrate, clear . Mount sections in DPX

RESULTS
Neurites around senile plaques, neurofibrillary tangles - Black.

farjam.md
farjam.md's picture
A QUESTION

A QUESTION
What is the Gold Chloride specified in method?
Is it AuCl (Gold (i) Chloride) or Gold(III)Chloride?