Acetylcholinesterase staining and immunofluorescence

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Fightforsight
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Acetylcholinesterase staining and immunofluorescence

 Hi,

I have brain sections that have GFP labeled axon tracts. Is it possible to do acetylcholinesterase staining on these sections without disrupting the GFP signal? Thanks!

Praseeda

Kristina Holmberg
Kristina Holmberg's picture
 Hello,

 Hello,

I would suggest that you try staining for both ChAT and VAChT, because in some systems one might work better than the other. If you look at biocomepare www.biocompare.com/Antibodies/ you will finde plenty of antibodies for the two markers. Personally I have used antibodies from Millipore and they have worked nicely in rat and mouse brain.

goat polyclonal anti-ChAT (catalog #AB144P) and goat polyclonal anti-VAChT (catalog #ABN100;). 

Best of luck!