NanoDrop Spectrophotometry

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Wigan
Wigan's picture
I have actually used all of

I have actually used all of them except the new ND2000C although I have seen one in operation
All the systems have their strengths and weaknesses so you need to decide what is most suitable for you and I share mol's frustration at the lack of science at times when people use instrumentation.  I know of a major research organisation that evelauted all the instruments and the NanoPhotometer came out tops but the Nanodrop is the market leader because most labs don't consider the options.
Try before you buy is the best option

The FFM
The FFM's picture
Wigan, do you have a link to

Wigan, do you have a link to that major research comparison of all the devices?  It would be a valuable read.

Cheers

mol
mol's picture
 i think it is a little

 i think it is a little unfair to say that nanodrop are only there because people don't consider the alternative. Granted, i've tried the nanophotometer in the past and there isn't anything wrong with it. Performance-wise i found the NanoDrop better but if you just take the numbers, there isn't a whole lot in it. NanoDrop is number one because it does things differently - i know you like them but cuvettes suck (kinetic assays excluded - although we have a plate reader for this). If they are disposable then they are expensive. If they aren't, then they are a pain to clean. If i've got 20 or 30 samples it is only 5 or 10 mins work on nanodrop. It takes that to clean a cuvette properly! 

BTW - may have need to go up in throughput soon. Anyone used any of the microplate, low volume products? I know Tecan have one and have seen an advert for a biotek version. Any others to consider? 

The FFM
The FFM's picture
 The Nanodrop 8000 has a 96

 The Nanodrop 8000 has a 96 well format

Wigan
Wigan's picture
Analytic Jena have a muliti

Analytic Jena have a muliti channel system although rumour has it that it is not robust.
Tecan seems to be popular and Thermo have launched a new system called multiscan which include DNA applications but not had a look and it may be single cell rather than 96 well analysis.
BMG may also worth checking out as they make reliable systems

SoltanloH
SoltanloH's picture
Hi folks, I am going to

Hi folks, I am going to purchase one of these instrument for my lab.
I read lots of forums about Nonodrop, Nanophotometer and pikodrop.
also, I got consaltant from other institue/lab are using them. I finally consested to buy Nanophotometer from Implene. I will post any satisfactory/dissatisfactory after using it.

Hassan Soltanloo, Ph.D.
Assistance Professor of Molecular Genetics and Genetic Engineering,
Department of Plant Breeding and Biotechnology,
Colleges of Agricultural sciences,
Gorgan University of Agricultural Sciences & Natural Resources,
Gorgan, IRAN.
Tel: 98-171-4426813 Ext 266
Fax: 98-171-4420981
Email: Soltanlooh@gau.ac.ir

quantum1361
quantum1361's picture
Hey all.  I just stumbled

Hey all.  I just stumbled upon this thread.  BioTek makes a very cool product that is standard 96 well microplate reader but has an adpater that essentially turns the reader into a 16-channel nanodrop.  All for around the same price of a single channel nanodrop.  It's called the Epoch/Take3 system.  Our lab just got one and we never even use our nanodrop anymore.

http://www.biotek.com/products/microplate_detection/epoch_multi_volume_spectrophotometer_system.html

SamplePrepGuy
SamplePrepGuy's picture
Has anyone seen the new UV

Has anyone seen the new UV/VIS Spetrophotometer LabChipDS by Trinean/ Caliper LS?  It uses microfluidic chips to create a miniature (1ul sample) cuvette, and is in 96 well format.  The chips are super cheap- $0.25/ sample.  I've been waiting for a product like this forever.  Let me know if anyone has tried this, I am scheduling a demo now.
http://www.caliperls.com/products/labchip-ds.htm

mol
mol's picture
 

 
quantum1361 - looks interesting. so how long does it take to do the full spectral scan on all 16 samples? Looks like it is fixed pathlength so do you know what the concentration range for e.g. DNA is?

SamplePrepGuy
SamplePrepGuy's picture
The literature I have says

The literature I have says under 6 minutes for 96 samples- so I'm guessing that breaks down to about a minute per 16 samples.  It looks like there is a single and dual pathlength chip available with the dual pathlengths of 0.2 and 1mm and a single pathlength chip of 0.5mm.  The range of DNA it quotes is 5- 3000ng/ul, pretty good for my application which is quantification following extractions. The cool feature which isn't available yet (supposedly sometime later this month)  is an analytical software features that interprets the spectrum and provides information on contaminating species present (i.e. if you want to measure the leftover protein from your DNA extraction) I attached the app note I got from my local sales rep...

mol
mol's picture
 I assume the six minutes is

 I assume the six minutes is for dual wavelength? I watched the video on the website and I have a biotek reader and unless they have had some breakthrough in speed, this isn't for a spectral scan (the epoch is single channel right?). I think 6 minutes is probably pushing it to load samples, read, eject, clean and reload samples times even if just using 2 wavelengths.  Saying that, in a typical Biotek way, they have copied technology from elsewhere and made a reasonable job of reproducing it. 

SamplePrepGuy
SamplePrepGuy's picture
Sorry Mol- the six minutes is

Sorry Mol- the six minutes is for the Trinean DropSense/ Caliper Labchip DS.  The scan itself is what is quoted at 6 minutes.  There's no cleaning of pedestals as it utilizes disposable microfluidic chips.   

tporrata
tporrata's picture
Hi!  We have a NanoDrop 2000

Hi!  We have a NanoDrop 2000 available here at our Molecular Biology Core Lab.  Our users love this equipment as it has many conveniences and it is user friendly.  However, I have to agree with previous post were 1ul of sample is quite variable.  As for protein, two of our users, with vast Bradford experience have tried it and have not been able to make it work due to its high variability within the standard replicates. 

Shazaam
Shazaam's picture
The NanoDrop is a waste of my

The NanoDrop is a waste of my samples and time. I won't waste a dime on the piece. It's overrated! My lab has two nanophotomters, the Peral and the NanoVue, and we got a great deal from our Denville Scientific Representative. I can give you his contact info, and he'll hook it up, and let you demo both at the same time for comparison purposes.

mol
mol's picture
 lol - thanks to the Denville

 lol - thanks to the Denville Scientific (who??!!) rep there

Guest (not verified)
Guest's picture
We compared these

We compared these nanospectrophotometers in 2009 with regular Agilent HP8453 and Beckman DU650 spectrophotometers using nucleic acid samples. The nanospectrophotometers have larger errors - they are good for molecular biology, but inaccurate for physical chemistry (binding, kinetics, etc). Regular spectrophotometers were reproducible within 0.5% of absorbance or better, nanospectrophotometers had errors several percent up to 10%.

Raashmi
Raashmi's picture
Hi,

Hi,

I have extensively used Nanodrop Spectrophotometer n it works really well.

The accuracy of the instrument is also really good. I have used it on a daily basis and the maintenance cost is also very less.

I will highly recomend this instrument.

Thanks

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