Nanodrop data

6 posts / 0 new
Last post
nix-j-c
nix-j-c's picture
Nanodrop data

Hi, i'm a biology student and am writing a report about studying organisms that live deep in the ocean.

I was wondering if the nanodrop machine could be used to analyse samples from such organisms, and what data the machine gives you, and what can be deduced from this.

Thanks for any help, I've seen that there's another thread about the nandrop in general, but it doesn't ask for this kind of information so I couldn't see any replies including such info.

 Thanks, Nix.

Hoepfully i've posted this in the right section.

The FFM
The FFM's picture
 You need to tell us what you

 You need to tell us what you require it to do so that we can reply whether it can do it.

What do want to measure? What is in your samples?  What assays will you be running?

Absorbance at 1 or more particular wavelengths? The Wavelength range of the Nanodrop 2000 is 190-840 nm.  So if you application required reading absorbance in that range then, yes it may well be useful for you.

If you need to measure fluorescence rather than absorbance the Nanodrop 3000 has UV, blue and white excitation and measures fluorescence ranging from  400-750 nm

nix-j-c
nix-j-c's picture
Eeerrr, could you tell me a

Eeerrr, could you tell me a bit about both, it dpeends on which of those variables I can ge the most information about and if I can say if the information can tell you a lot about the creature.

Biju
Biju's picture
Hi

Hi
Nanodrop could be used for measuring concentration of DNA , RNA or protein isolated in pure form from samples. It is mostly used for DNA and RNA only. The typical volume used is 1 ul  and gives output as ng/ul of sample for DNA (single or  double stranded)  and RNA.
Biju

Dr._Eldon_Tyrell
Dr._Eldon_Tyrell's picture
  You want to measure DNA and

  You want to measure DNA and/or Protein of deep-sea creatures.. What for?
I mean, protein measurement by Nanodrop is not extremely precise (good enough for a rough estimate) but why would someone do this?
The Nanodrop is great for determine DNA, but same question here: Why measure DNA of deep-sea organisms?

Just that you get that right, the Nanodrop is made for analysis of more or less pure samples.
For example: Measuring plasmid-DNA extracts from E. coli or purified recombinant protein from yeasts.

You should tell us what you're trying to show with these measurements and then we could tell you whether the Nanodrop is a suitable device..

What are we even talking about? Deep-sea fishes? crabs? worms?
 

nix-j-c
nix-j-c's picture
I'm doing coursework on the

I'm doing coursework on the problems of studying deep sea organisms and found the nanodrop technology and did a bit of research and found that it's used on deep sea organism DNA, as I'm focusing on what DNA analysis can tell a scientists about the organism. (Just general deep sea organisms I supose)