THP-1 Xenograft development in mice

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manderson
manderson's picture
THP-1 Xenograft development in mice

I am seeking input on devloping a THP-1 ( acute monocytic leukemia) disseminated and/or solid tumor xenograft model in mice.
mouse strain choice is my question
My only reference found ( so far) shows the use of SCID + anti-asilo GR1 + irradiation.  My question is if a NOD/SCID could be an effective substitute? 
 SCIDhu is an option, though a less desireable one due to expense time and labor.  NOD/SCID gammaKO could be considered, but again a less desirable ( more expensive) choice.  I would choose NSG KO over SCIDhu if that were the choices
Thank you for any input or leads you can offer
 
 

marcus muench
marcus muench's picture
I've been comparing human

I've been comparing human hematopoietic engraftment in NSG over NOD-SCID as my lab move to this new model and I have to say I much prefer the NSG.  I have no experience with the leukemia your studying, but my guess is that NSG is your best mouse.  SCID-hu offers the humanized environment, which has its pluses, but maybe you don't need that.  
BTW, the NSG breed well in our hands- many litters over 10 pups, so you can cut down on costs by breeding your own.