thioglycollate excretion--help!

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mmavers
mmavers's picture
thioglycollate excretion--help!

we are preparing our animal protocol and the animal facility wants to know if mice injected IP with 4% aged thioglycollate will excrete it and therefore if it will be harmful to employees. does anyone know the answer to this (and even better have a citation to back it up)? thanks!

Sangram
Sangram's picture
Most important hazard: Not

Most important hazard: Not classified as hazardous.
Health hazard > Inhalation: Moderately irritating to the respiratory system.
Health hazard > Skin: May cause irritation.
Health hazard > Eyes: May cause slight transient irritation.
Health hazard > Ingestion: May cause irritation.
also see the attachment

http://ntp.niehs.nih.gov/index.cfm?objectid=073130CE-FC29-17D9-4872CBC87585FA28
[b]

mmavers
mmavers's picture
thanks for your help and your

thanks for your help and your quick reply. however, i was of the understanding that sodium thioglycolate is different than thioglycollate. specifically, we are using a solution of brewer thioglycollate medium (sigma #B-2551). if anyone knows about the excretion of this product (or similar products from other companies) after IP injection, i'd appreciate the info. thanks!

Sangram
Sangram's picture
please just email sigma !

please just email sigma !
they will provide you the detail biosefty guidelines!
thank you!

mmavers
mmavers's picture
that was the first thing i

that was the first thing i did! they said they don't test any of their products for specific applications (esp. animal studies), so they have no idea.

Omai
Omai's picture
I'm moving this discussion to
Jen_Floyd
Jen_Floyd's picture
I regularly inject mice with

I regularly inject mice with thioglycolate to elicit macrophages and there is nothing in our protocol to indicate that it would be secreted. We're normally injecting only 1 ml per mouse and that is enough to get plenty of cells.

R Bishop
R Bishop's picture
Examples of accepted IACUC

Examples of accepted IACUC protocols on this topic

http://www.iacuc.ucsf.edu/Policies/awSPThio.asp

http://www.bu.edu/research/compliance/oversight-committee/iacuc/policies/thioglycollate.shtml

Sorry dont know anymore about the excretion rate but these may help get you through IACUC at your Uni