mouse strain characterization

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ventresurpieds
ventresurpieds's picture
mouse strain characterization

Hi,
I'm working on mixed background (129 & C57Bl6) mice and would like to find out the percent of each strain in my mice exactly. Does anyone know of any kit or methods that I could use?
Thanks very much in advance!

Arvind Singh Pundir
Arvind Singh Pundir's picture
Background strain
Ivan Delgado
Ivan Delgado's picture
Hi ventresurpieds,

Hi ventresurpieds,
You may want to consider a service like the ones provided by KBiosciences. Their speed congenics service will provide you with the most detailed information you can obtain regarding the strain composition of your mice.
 

ventresurpieds
ventresurpieds's picture
Thanks for your replies and

Thanks for your replies and suggestions, I have been in touch with a number of companies for price quote.
The mice that I work with are from inbred colony and I'm only interested in contribution of the two background strains. Is it necessary to have 100+ marker panels tested?

Ivan Delgado
Ivan Delgado's picture
 

 
I have very limited experience with this, but the bottom line is this: the more genome coverage, the better your data will be, and as a result the fewer crosses you will need to perform. As for where the law of diminishing returns begins to have an effect, I am not sure. 100 marker panels are not extensive, so my guess is that you would still be getting the benefits of extra data for your money. 
I would ask the companies you are talking to for an estimate of how much faster to >99% inbred you can get with their products. They typically have nice side by side comparisons of inbreeding schemes with and without their technologies.