Animal tail vein injection techniques

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biolearner
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Animal tail vein injection techniques

It's a very useful, but difficult way, to give agent via animal (especially mouse) tail vein injection. The below are some point and atlas to fulfill this procedure. Remember! There are totally 3 veins in a mouse tail, so you still can inject if you failed once, even twice.

http://www.lab-manual.com/lm_383.htm

R Bishop
R Bishop's picture
Having a done a ton of these

Having a done a ton of these this year, I can tell you there are no simple ways to learn this technique only practice, practice, practice.

Thanks for the protocol.

Rb

ramesh padodara
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Exactly I agreed with you,

Exactly I agreed with you, but one can do this performance in one trial also, simply it required concentration and restrain of animal.

g a
g a's picture
CAN YOU PLEASE GUIDE ME AS

CAN YOU PLEASE GUIDE ME AS PER OW MUCH VOLUME YOU CAN INJECT THROUGH TAIL VEIN IN MICE AND RAT BOTH?
THANKING YOU IN ANTICIPATION
GAGANJOT SINGH

tbiggie
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Does anyone know what the

Does anyone know what the maximum volume of blood that you can withdraw from the tail vein is?

R Bishop
R Bishop's picture
[Argerine,

[Argerine,
 
The max you can inject is 200ul into a mouse.  I have a lot better luck with 100ul.
Rus

R Bishop
R Bishop's picture
tbiggie,

tbiggie,
 
What organism?  For a mouse,  I think the limit is 150ul per day, but that would be really hard work to get.  I reccomend eye bleeding, its easy, non-painful, and you can easily get 200 ul per mouse.
 
Rus

Arvind Singh Pundir
Arvind Singh Pundir's picture
Practical blood sample

Practical blood sample volumes for laboratory animals, domestic species and non-human primates* are given in the link  : www.nc3rs.org.uk/bloodsamplingmicrosite/page.asp
*(adapted from Wolfensohn & Lloyd, 2003, Handbook of Laboratory Animal Management and Welfare, 3rd Edition)

Arvind Singh Pundir
Arvind Singh Pundir's picture
For   blood sampling from rat

For   blood sampling from rat, mouse, guinea pig, ferret, dog, pig rabbit etc. related querries and to choose the most appropriate technique refer :   www.nc3rs.org.uk/bloodsamplingmicrosite/page.asp

g a
g a's picture
Thanx alot Arvind

Thanx alot Arvind
I could not find the optimal volume for injection or isolation from mice which is being provided in the link you posted.

heehawmcduff
heehawmcduff's picture
Thank you - that web site is

Thank you - that web site is incredibly useful and even includes Ferrets.  If you click the links on the left as well it includes details and instructions of some common techniques used such as cardiac puncture or cannulation.

scifigr8
scifigr8's picture
here is a link, u can follow

here is a link, u can follow as it is.
u can give injecton under restraint or anaesthesia.
confirm the i.v inj by  blanching of the vein and not aspiration of blood, its imp.
www.theodora.com/rodent_laboratory/injections.html
 
best wishes

Rebeca
Rebeca's picture
 There are 2 veins (laterals)

 There are 2 veins (laterals) and 1 artery (at the top); careful here because it is not the same. But yes, you have several chances to get it right; you should choose first one of the 2 lateral tail veins and start your first try as far away from the "rear end" of the mouse as possible. If you fail, you can try again a little bit closer to the body and so forth.  If you still can't get it, you can start with the other lateral tail vein. This method gives you about 6 chances per mouse.
You should inject your compound as slowly as possible and do not use a volume higher than 200ul or the 10% of the body weight of the animal (whatever is smaller). Be very careful with the bubbles; the tiniest one will kill your animal. 
I hope this help.

kikidee
kikidee's picture
Not to contadict or confuse

Not to contadict or confuse anyone, but there are actually 3 veins in the mouse tail and one artery. Their specific locations are the 2 laterals running along each side of the tail; the vessel on the TOP of the mouses tail is actually the DORSAL VEIN while the vessel on the ventral (bottom) portion af the tail is the VENTRAL ARTERY . The dorsal vein is not commonly used for injection due to it being more difficult to access for injection- BUT CAN be used!

Rebeca
Rebeca's picture
 You can inject up to 200

 You can inject up to 200 microlitters (10% of their body weight) per day. If you are injecting something that you are not sure about the potential toxicity, I wouldn't inject them more than once every 3 days... 

Rebeca
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 Upss!! That was for mice,

 Upss!! That was for mice, but I am quite sure the 10% body weight rule applies for rats too.
Good luck

Rebeca
Rebeca's picture
 Oh, great!! I didn't know

 Oh, great!! I didn't know about the 3rd vein; I was always told that my only options were the 2 laterals. Very useful knowledge indeed. Thanks Kikidee