latest transformation techniques which save time

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amits
amits's picture
latest transformation techniques which save time

hi i am working on producing stress tollerence in crops in that i have cloned few stress tollerent gene n trying to clone few more
but genral transfomation techniques takes a day  but now a days  i a m using a kit (ZYMO)base compitant cell preparation n transformation  which save  so much time it take  onlu 10minutes   but i want to know bit advance techniques which is much more efficient in cloning
iam waiting for your ideas