quantification of stainined histology photos using Image J software

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nalpy
nalpy's picture
quantification of stainined histology photos using Image J software

Dear all

I used image J software for quantification of immuno blot . please i need systamatic procedure to use image J software in to quantify stainined ( coloured) histology photographs to provide values. if i use which parameters i selected in image J? I need to calulate length of basement membrane in electron microscopic photos using this software? pls help me to solve my problems.
Thank you to all

Guy Sovak
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If no one will help you with

If no one will help you with image J,  try using another free image analysis software jMicroVision.
Just type the name and you will get the web site . With this I can help you .
Guy

nalpy
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Dear gsovak

Dear gsovak

Thank you for kind help. I download and ask the details steps to stained area to quantify histology photos
kind regards
palanisamy

nalpy
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Dear gsovak

Dear gsovak
I download Jmicrovision. pls i need your help to quantify coloured or stained part of histology photos and take measurement for distance  between membrane thickness in electron microscopic photos. ineed your mail id also.
regards
palani

20101975
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Try for the

Try for the StereoInvestigator 8 or 9. thats very good. Let many things can be done there. Its realy very good.

nalpy
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Thank you sir. but it could

Thank you sir. but it could not download free version. pls help me.
regards
palani

Guy Sovak
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Hi Palini.

Hi Palini.
Please let me know what do you want to quantify?
Guy

nalpy
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Dear Sir

Dear Sir

Thank you for your replay. I download Jmicrovision1.2.7. I try to apply the tools to some application. I need to quantify staining area of my histology and immunohisto photos and measure the thickness width , length in nm scale in electron micrograph photos.
I need systamatic flow chart explanation the way i use the software.
thank you
regards
palani

Guy Sovak
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Hi,

Hi,
First step is to do calibration .
You can find a lot of information under help in the software itself.
If you can upload one image that you want to quantify and tell me exactly what you want to do I can help you .
Guy

Roderick
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Hi Guy,

Hi Guy,

I tried to PM you but the website kept referring me to somewhere else instead of a possibility to pm you. I've noticed that you are skilled in quantifying stained histology images. Currently I am trying to find a solution to quantify stained slices of intervertebral discs. ImageJ helps me a little but I don't seem to get good results with it. I hope you can help me!

Thanks in advance.

Roderick
(medical student)