I am keen to measure the fluorescence intensities of two images taken by using threshold method of analysis by Image fluorescence. How could I measure the fluorescence intensity (pixels) of any image and can compare with other images taken under the same camera settings and fluorescence by using ImageJ? Which application of ImageJ would be used for images captured for red (single) channel? Do i need to convert the images in 8-bit types or black and white before measuring?
And also is it possible to measure from region of interest or for whole image?
Hey guys, can you help me out!!