Immunogold staining

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gsovak
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Immunogold staining

Hi all,
Dose any one have an expireance with triple staining, using three different sizes of gold particles?

jonatmudd
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I don't have a lot of

I don't have a lot of experience myself, but it looks like there is a good reference (listed below). They describe using 3 sizes of gold that decorate membranes and are clearly distinguishable. Their sizes for gold were 5, 20 and 40nm.

Journal The Histochemical Journal

Volume 21, Number 3 / March, 1989/136-144

Good luck!

gsovak
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Thanks for the information

Thanks for the information
Guy