Viewing GFP in whole mount ovules of Arabidopsis

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srbhat22
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Viewing GFP in whole mount ovules of Arabidopsis

I am screening promoter trap lines of Arabidopsis carrying GFP to find promoters specific to different cells (egg cell, nucellus, central cell etc) within Arabidopsis ovule. I would like  to use confocal microscope and whole ovules (without sectioning) .  I find that ovule clearing using Hoyer's medium destroys GFP. Please suggest a way to visualize internal cells expressing GFP.