imaging of macrophages using confocal microscopy

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lakshman
lakshman's picture
imaging of macrophages using confocal microscopy

hello everybody,
i am interested in imaging mouse peritoneal macrophages using acridine orange. can i get some suggestion regarding the wavelenghts to be used.

Richard Taylor
Richard Taylor's picture
Complex

Complex Excitation -> Emision (nm)

Acridine Orange + DNA 502 -> 526

Acridine Orange + RNA 460 ->650

Acridine Orange, both DNA & RNA 440-480 -> 520-650

Source

Information on Acridine Orange alone, which corresponds with the above:
http://omlc.ogi.edu/spectra/PhotochemCAD/html/acridineorange.html

You may be best off finding your own optimal wavelenghts, if that's practical for you.

lakshman
lakshman's picture
thank you Mr. Taylor. shall

thank you Mr. Taylor. shall try to optimize the wavelength.