Golgi marker

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jenny99
jenny99's picture
Golgi marker

Hi!

I'm looking for an antibody directed against a protein resident in golgi vesicles. Any suggestions? I want to use it in immuno fluorescence and western blot.

Thanks

Guy Sovak
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Hi

Hi
People in my lab used TGN46 for immunostaining, and it works perfectly.
It isnt good for WB.

Fraser Moss
Fraser Moss's picture
You should try and obtain a Y

You should try and obtain a Y/CFP-tagged version of the N-linked glycoprotein-processing enzyme galactosyltransferase (GalT)

example of its use:

Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities.
Mol Biol Cell. 2006 Feb;17(2):990-1005.
http://www.molbiolcell.org/cgi/content/full/17/2/990

actual cloning paper:

Sciaky, N., Presley, J., Smith, C., Zaal, K. J., Cole, N., Moreira, J. E., Terasaki, M., Siggia, E., and Lippincott-Schwartz, J. ((1997). ). Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J. Cell Biol. 139, , 1137-1155

http://www.jcb.org/cgi/content/abstract/139/5/1137?ijkey=08441ef46a93adb18848dc6a4c94236b30f7caf9&keytype2=tf_ipsecsha

If you still need to do immunotechniques you can use the anti-GFP antibodies that are commercially available.

Any paper from the Lippincott-Schwartz lab should help you out because she is so preminent in this field.

jenny99
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Thanks.

Thanks.

This will help me along!

cdnsciguy
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jenny99 wrote:Hi!

jenny99 wrote:

Hi!

I'm looking for an antibody directed against a protein resident in golgi vesicles. Any suggestions? I want to use it in immuno fluorescence and western blot.

Thanks

Hi Jenny, I would contact someone at the glycobiology program at the university of california in san diego. Since it is a golgi protein (and the golgi is heavily involved in glycosylation) i thought that someone there might be able to help...

good luck!

k.