Giant Pseudomonas aeruginosa mutants

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drtaing
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Giant Pseudomonas aeruginosa mutants

It was really a strange discovery.
When P. aeruginosa was treated with yeast fermented broth for prolonged time, most cells died but mutants survived. They were as 100 times long as wild type; they didin't swim. Some of them produced reddish brown pigment. I have transferred them on agar slants several times for more than 3 years but most of them didn't lose their phenotype properties.
I can share my mutants to collaborate a project inquirng on the giant cells.
PS: I attach microphoto of a singel mutant cell.

Dr. Taing Ok, Kagoshima University, Japan
eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%64%72%74%61%69%6e%67%6f%6b%40%79%61%68%6f%6f%2e%63%6f%6d%22%3e%64%72%74%61%69%6e%67%6f%6b%40%79%61%68%6f%6f%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))

jachmoody
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How do I see the photo you

How do I see the photo you mentioned--didn't see an attachment?

jim

ps.
if you want send reply to eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%6a%61%63%68%6d%38%38%38%40%79%61%68%6f%6f%2e%63%6f%6d%22%3e%6a%61%63%68%6d%38%38%38%40%79%61%68%6f%6f%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))

thanks,
jim

jachmoody
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[Got the email with the

[Got the email with the photomicrograph--really fascinating!

Were the yeast treatments that induced the mutants done in broth with double the typical concentrations of carbon and nitrogen source--so as to reduce cell wall lysis?

jim

quote=drtaing]It was really a strange discovery.
When P. aeruginosa was treated with yeast fermented broth for prolonged time, most cells died but mutants survived. They were as 100 times long as wild type; they didin't swim. Some of them produced reddish brown pigment. I have transferred them on agar slants several times for more than 3 years but most of them didn't lose their phenotype properties.
I can share my mutants to collaborate a project inquirng on the giant cells.
PS: I attach microphoto of a singel mutant cell.

Dr. Taing Ok, Kagoshima University, Japan
eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%64%72%74%61%69%6e%67%6f%6b%40%79%61%68%6f%6f%2e%63%6f%6d%22%3e%64%72%74%61%69%6e%67%6f%6b%40%79%61%68%6f%6f%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))