HI TO ALL,
I HAVE INFECTED SOME MONOCYTES WITH TB(in vitro assay). I now want to confirm that i've infected these cells using REAL TIME PCR.
Does any one have protocols as to the best DNA extraction method (keeping in mind that lysing of the bacterial wall is more difficult versus host cell membranes).
Once the DNA has been extracted, how can i separate the host's DNA from that of the bacteria's???
NOTE: need these results ASAP, all suggestions welcome.