multiplicity of infection

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PriyaSar
PriyaSar's picture
multiplicity of infection

hello

a suspension of temperate phage contains 10^7 particles/ml, only  10 % are capable of infecting.If 100 microliters of this  phage suspension is mixed with 1000 bacterial cells. what will be the multiplicity of infection?

how to calculate moi..any formula is there..?

Megaviswanath
Megaviswanath's picture
MOI — The MOI is the number

MOI — The MOI is the number of viral particles per cell that is desired during the infection reaction. The optimal MOI may have been determined for a particular cell line already and if so just utilize that number for all AdenoSilence viruses. If not, then a range of MOIs (e.g. 50, 250, 500, 1000, and 2500) will need to be assayed in an attempt to identify the optimal infection amount. The optimal MOI should display a high transduction efficiency with low cellular toxicity. Most MOIs will fall in the range of 100–2500 but they can be higher or lower.

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