Biologist Postdoctoral Fellow (JBEI) – 80336
Organization: PB-Physical Biosciences
The Fuels Synthesis division of the Joint BioEnergy Institute (JBEI) has an opening for a Biologist Postdoctoral Fellow with experience in anaerobic and molecular microbiology to engage in research on novel pathways of bacterial biosynthesis of fuel constituents. The successful candidate will participate in research to identify genes associated with biosynthesis of novel biofuel molecules from anaerobic bacteria using protein purification, bioinformatic analyses of metagenomic and proteomic data, heterologous expression of candidate genes in E. coli and assays of relevant activity, and other
• Cultivate an existing anaerobic bacterial community for use in biochemical studies (protein purification, in vitro assays).
• Partially purify the target enzyme by FPLC under anaerobic conditions using anaerobic, in vitro assays.
• Assist in the identification of candidate fuel biosynthesis genes based on proteogenomic analysis of active FPLC fractions.
• Express the candidate genes heterologously in E. coli and perform in vitro assays for the target activity; troubleshoot heterologous expression as necessary.
• Participate in the preparation of manuscripts for publication and in the development of funding proposals and patents.
• Participate in group meetings and seminars.
• Perform research lab procedures efficiently and with attention to necessary quality control procedures.
• Maintain well-documented laboratory notebook (e-notebooks will be used).
• Additional – Support in vitro assays by performing analyses by liquid chromatography/mass spectrometry.
KEY SUCCESS FACTORS:
• Recent Ph.D. in Microbiology, Biochemistry, Molecular and Cell Biology, or a related field of science.
• Experience in the cultivation of anaerobic bacteria and use of an anaerobic glove box.
• Extensive experience performing laboratory research using standard techniques of molecular biology, including extraction and manipulation of RNA and genomic and plasmid DNA, gel electrophoresis, PCR (including qPCR), and cloning.
• Experience with performing in vitro, biochemical assays.
• Good background in biochemistry.
• Familiarity with common bioinformatics tasks (e.g., BLAST searches).
• Ability to work with guidance from a director or principal investigator and collaborators and well as to conduct experiments independently, maintain appropriate records, and manage data.
• Excellent written communication skills along with good organizational and interpersonal skills.
• Ability to work in a team environment and effectively interact with a broad range of colleagues.
• Experience with heterologous expression of genes in E. coli under anaerobic conditions
• Experience with protein purification, particularly under anaerobic conditions.
• Experience with gas chromatography/mass spectrometry and/or liquid chromatography/mass spectrometry.
• Experience with analysis of next generation (Illumina) genomic/metagenomic data.
• Experience with standard protein biochemistry techniques (such as SDS-PAGE, Western blots).
NOTES: This is a 1 year term appointment with the possibility of renewal for up to 5 years based upon satisfactory job performance, continuing availability of funds, and ongoing operational needs. Salary for postdoctoral positions depends on years of experience post-degree.
This position requires completion of a background check.
How To Apply
Apply directly online at http://18.104.22.168/counter.php?id=29251 and follow the on-line instructions to complete the application process.
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