I was reading about IVET and I wonder how they mutate a certain bacteria so that it lacks a certain gene (from wich you want to research the promotor).
How can you influence the chromosome of that bacteria so that the gene that you want to delete is removed from that bacteria.
I know they use a plasmid for this but how does this work? Does this plasmid then have all the same dna (chromosome) as the bacteria expect the gene you want to delete or?
how to create an auxotrophic bacteria