comparing protiens at the surface

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Nazunderwent
Nazunderwent's picture
comparing protiens at the surface

well i am looking for somone who would help me for identification of some virulance factors that may present at N.meningitites bacteria 
i would compare some protiens from this strain and another bacterial strains that might have similar genatic make up  so 
i f some one have done that and would like to help in ideas for this topic it would be greatful. ?
thanks  

Chin Fen Teo
Chin Fen Teo's picture
Hi Nazunderwent,

Hi Nazunderwent,

I might be hand-waving here, but one of the approaches comes across my mind is outlined as follow-

(1) Biotinylated surface proteins with NHS chemistry
(2) Strepavidin (or avidin etc) pull down
(3) Mass-spec ID

Good luck!

Nazunderwent
Nazunderwent's picture
thanks alot pippiri

thanks alot pippiri