exopolysaccharide

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MJ1987
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exopolysaccharide

Hi,
      I am Mayur Joshi..I am working with exopolysaccharide production from Bacteria as well as from White rot fungi. I want some information regarding viscosity improvement under submerged condition.Extractin, Estimation of exopolysacchaide.So, please help me out by poviding the information as above mentioned problems.

Lynn E.
Lynn E.'s picture
We did a pprecipitation

We did a pprecipitation/purification of extracellular polysavccharides using cetyltrimethyl ammonium bromide.  Cells were washed and centrifuged several times.  The supernate was filetered prior to the precipitation step.  If I recall, the precipitate was then dialyzed against a solution of sodium chloride to remove the CTAB.  Here is a link to one paper that might be helpful-

We were able to quantify to some extent using the Anthrone assay.  One group broke the chains into their constituent sugars and derivatized (silanization?)for analysis by GCMS

http://chemistry.unpad.ac.id/isc-proceeding/2008/Pdf/OP/0294-0296%20OP075%20-%20Acep%20Riza.pdf