An assay to confirm bacterial structure

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angelaevan
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An assay to confirm bacterial structure

Hi,

We have been immunizing with live Rhodococcus equi (gram positive) to elicit an immune response, we show show that works in raising IgG levels for that antigen with Elisas.  Now we could not use the live R.equi and we "attenuated" it with UV irradiation (254nm)  we are getting good elisa results and have even done western blots of the serum comparing the serum from live antigen and the irradiated antigen and are getting similar bands.  But,  we need to show that the bacteria has remained structurally intact does anyone know how I can do this?