black holes on array

1 post / 0 new
Scot E. DOwd Ph.D.
Scot E. DOwd Ph.D.'s picture
black holes on array

using a microarray opteron we have a large number of spots that looked good with neutral dye. upon running through the entire hyb these spots appear as devoid of color. Not even the background is showing on these spots? what the hey? anyone experience this? almost like quenching or complete blocking?