Hand held 96-well pipetter?

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steven_a_jones
steven_a_jones's picture
Hand held 96-well pipetter?

I am trying to find a hand held 96-well pipetter. Does anyone have any ideas where I might find one? I have seen the robotic plate pipetters, but I do not feel they will work for my application. Thanks for any help.

Roshan
Roshan's picture
steven_a_jones wrote:I am

steven_a_jones wrote:

I am trying to find a hand held 96-well pipetter. Does anyone have any ideas where I might find one? I have seen the robotic plate pipetters, but I do not feel they will work for my application. Thanks for any help.

Are you looking for a used one or you want to buy a new pipetter? I do not recommend used. Let us know the capacity and the usage.

Thanks,
Roshan

steven_a_jones
steven_a_jones's picture
A new one. Fixed or

A new one. Fixed or adjustable volume, some where around 10ul. Thanks for your reply!

Roshan
Roshan's picture
steven_a_jones wrote:A new

steven_a_jones wrote:

A new one. Fixed or adjustable volume, some where around 10ul. Thanks for your reply!

The best company to contact is : VWR International at
800-932-5000. They have all kinds of variable and fixed multichannel pipettors.

Good luck,
Roshan

Simbiotix
Simbiotix's picture
steven_a_jones wrote:I am

steven_a_jones wrote:

I am trying to find a hand held 96-well pipetter. Does anyone have any ideas where I might find one? I have seen the robotic plate pipetters, but I do not feel they will work for my application. Thanks for any help.

Steven automated pipettors can be very versatile in what they can do especially when add on peripherals are used. For example you could add a tilting nest to the pipettor so that you get all the serum out of the well. If you wish we can discuss your application and I can see what Simbiotix can do about providing you a solution.

Paul Weinreb
Paul Weinreb's picture
Steven - we've got a 96 well

Steven - we've got a 96 well pipetter that we ordered via Fisher; I can check on the brand next time I'm in lab and post later. I don't think the capacity goes down as low as 10 uL, however - it is probably more of a P200 range. We haven't used it much yet so I can't vouch for how well it works.

I think a 96 well pipetter would be great, but the problem with these things is usually making sure that all 96 tips get seated properly. The one we have has a mechanism for jamming on all the tips by using a handle - it works like those old credit card imprinters, if that makes sense.

Let us know if you find a good one.

omid
omid's picture
pw_18 wrote:Steven - we've

pw_18 wrote:

Steven - we've got a 96 well pipetter that we ordered via Fisher; I can check on the brand next time I'm in lab and post later. I don't think the capacity goes down as low as 10 uL, however - it is probably more of a P200 range. We haven't used it much yet so I can't vouch for how well it works.

I think a 96 well pipetter would be great, but the problem with these things is usually making sure that all 96 tips get seated properly. The one we have has a mechanism for jamming on all the tips by using a handle - it works like those old credit card imprinters, if that makes sense.

I have found a great company that offer s lightweight programmable pipet-aid. these pipet aids can rapidli dispense aliquots with unsurpassed precision:
check Pipet-Aid Elite from Drummond scientific company
www.drummondsci.com

Let us know if you find a good one.

ggrifit
ggrifit's picture
Try this link to Corning,

Try this link to Corning, they do a good one that does 25 to 200 L in 5 L increments
[url=http://catalog2.corning.com/Lifesciences/ProductDetails.aspx?p=Accessori...(Accessories)&region=NA&language=EN&CatNo=7605]Link[/url]

Cheers
Gareth
I think I might buy one. Does anyone know how good it is?

spotty
spotty's picture
Hi, I am also looking for a

Hi, I am also looking for a 96 well pipettor but I couldnt find any on the fisher or vwr web sites. Please can you guys help and send me a link or two. The only one I could find following some searching was the Corning TRANSTAR, but it doesnt seem as versatile as I would like.

Thanks in advance Spotty

marcus muench
marcus muench's picture
I'm very satisfied with the

I'm very satisfied with the multichannel pipetters from Rainin. Their tips make a good seal with the pipette, which really helps with the accuracy.

http://www.rainin.com/

spotty
spotty's picture
Thanks for the link. We have

Thanks for the link. We have a whole bunch of Rainin 8 well multichannels which as you say are great. I am however struggling to find a 96 channel on their web site. Any help much appreciated.

Spotty

marcus muench
marcus muench's picture
Sorry, I read your post too

Sorry, I read your post too quickly.

I'm also somewhat familiar with the Corning Transtar. The model I played with must have been the original about 15 years ago. I don't know if they improved them much since. Nonetheless, the feeling I was left with was that lining everything up just right and using the required care in pipetting was more problematic than just using a 12-well pipette 8 times. Off course, this all depends on how many plates your dealing with. We tended to have about ten plates at a time, so the Transtar just ended up sitting in the corner of the lab and we used the 12-well pipetters. If I had 50 plates, maybe it would be a different story.

I'm sorry I'm not aware of any other 96-well pipetters, but there is always robotics:

http://www.labx.com/v2/spiderdealer2/vistaSearchDetails.cfm?id=2768913

Roshan
Roshan's picture
Pipetman Ultra Multichannel

Pipetman Ultra Multichannel
Designed with 96-well plates in mind, Pipetman Ultra Multichannel is particularly useful for molecular biology applications: PCR* sequencing, immunology assays, series dispensing into 96-well plates, etc. Used with Diamond Tips from Gilson's patented Rocky Rack system, this product will maximize performance and assure productivity.
* PCR process is covered by US patents owned by Hoffman-La-Roche, Inc.
Check the website www.gilson.com

TRMADR
TRMADR's picture
steven_a_jones wrote:I am

steven_a_jones wrote:

I am trying to find a hand held 96-well pipetter. Does anyone have any ideas where I might find one? I have seen the robotic plate pipetters, but I do not feel they will work for my application. Thanks for any help.

Hi Steve, so I used the Corning Transtar (we had about 3 in my previous lab) for about two years. The first time we evaluated it, it worked poorly, to be honest. Part of it was poor training and the problems we had were leaky tips, and the fact that you had to immerse ALL the tips fairly evenly to aspirate your sample. Sometimes if one had it canted or angled slightly you would fail to pick up your sample.

I worked at BD PMG and when they acquired Trans Lab I went over for a week for the purpose of acquisition and "transfer" of some of their hybridoma technology to our Hybridoma lab. They used these, and thus began my training into the tips and tricks for working it.

A couple points: as I recall you could transfer about 20-30 ul reliably but anything less than that was a little iffy. 80 to 100 ul generally worked better.

This is not a high precision device. It was used for transferring 20 plates of sup from growing hybridoma wells to our elisa plates for testing. This was more or less a simple qualitative assay in when throughput was more important than absolute accuracy. In our case, if one well got 80 ul but another well got 30 ul or less, it wasnt going to really matter for us--at least at that stage. But one DID have to be careful about bubble formation, and sometimes we had drips which were never good. With practice, this became less frequent, but we never eliminated the problem entirely.

Another point is that the cassettes are not cheap. I dont recall how expensive they were at the time, but this is not a device where you can substitite fisher, or some other knock-off tips for your pipettor as you might with a rainin pipettor. You have to purchase the cassettes. So if you are in a lab running on a tight budget, this might be a consideration. Additionally, you can not reuse the cassettes. They have a latex cover--a manifold if you will, that gets stretched out pretty quickly. So if you are transferring from one plate and splitting to two other plates, you are ok there, but dont think you will be able to rinse these out several times and re-use. I know--we tried everything.

All in all, it was a fair solution for our needs, but we were looking at medium throughput low cost solutions at the time, and in retrospect, I dont know how much cheaper this was, considreing the volume we were running at, and hte cost of the cassettes.

So, hope that helps. Good luck!

sadia
sadia's picture
I m sadia and also searching

I m sadia and also searching for the same specification of hand held 96 pipetter. I searched by V and P scientific but my specification is infact to get 96 or 384 pipetter which allows me to transfer 30-40 l compound+DMSO solution into the replica plate from mother plate. But you can see this web site. might be you find your interested application product
www.vp-scientific.com

Mesatida
Mesatida's picture
Hi everybody

Hi everybody

there actually is a solution to your problem, it's called Liquidator 96 and it is a MANUAL 96 WELL PIPETOR! You can do all the things you want and it really works. This product will be sold through RAININ instruments in the US and I can give you more details about it if you want or redirect you to the right person. I worked with the instrument and have seen many happy users so far. This is really what you want.

Working Range: 5 to 200 ul, 4 position operation, fully manual, top precision and accuracy!

http://www.pro-4-pro.com/en/Lab/Company-16032/index.html

Just let me know if want wore details.

Tony Rook
Tony Rook's picture
Simply because I was curious

Simply because I was curious as to what this Liquidator 96 looked like, I have attached an image of this device below...