Postdoctoral researcher to develop isothermal DNA amplification methods at

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Postdoctoral researcher to develop isothermal DNA amplification methods at

Lehrstuhl für Technische Mikrobiologie at Technische Universität München in Freising, Germany is looking for a post doctoral researcher for a 36 month project entitled Development of isothermal DNA-based user friendly systems for the rapid detection and identification of mycotoxin-producing and gushing-relevant Fusarium spp. in brewing cereals and malt Background
Massive infection of cereal crops by Fusarium spp. occur during the flowering season under favourable conditions of moderate temperatures and high humidity. In addition to yield loss, these fungi influence the quality of brewing barley and malt in a variety of ways. Fungal proteases and amylases may lead to changes in grain composition and colour and toxic fungal metabolites, mycotoxins, pose a health risk for consumers. Moreover, some species may alter the composition of foam active proteins and induce gushing (over foaming of bottled beer) in beers produced from infected brewing malt.
Since Fusarium spp. are virtually insensitive to fungicide treatment and resistant cultivars are currently not available, early identification of infected lots during quality control is the only possible way of excluding infected lots from the brewing process. Besides classical microbiological methods, species and group specific PCR-based methods were developed at our institute during recent years. However, sample preparation and the necessity of a highly equipped PCR lab are still obstructive to the wider application of such methods in the brewing industry. Recently developed methods which make use DNA amplification under isothermal conditions for may lead to a substantial analytical simplification and increase of rapidness, here.
 
Research plan

During the current project, methods will be developed on the basis of isothermal DNA amplification methods (LAMP) for the sensitive and specific identification of quality relevant Fusarium spp. in sample materials relevant to the brewing industry. Assays shall be rapid and user friendly with a minimum of sample preparation. The project will benefit from the highly relevant experience, both in mycology and development of molecular detection assays which our institute has accumulated over the last 15 years.
 
Qualification

Successful applicants for this position have finished their PhD or equivalent graduation in a related area of research. They should have a background in either biology, biochemistry, molecular biology or in a technical discipline, e.g. food technology, with a clear molecular biological of biochemical specialization. The full-time position is restricted to 36 month. Applicants with a recently finished PhD graduation will preferably be able to obtain basic professional research experience and to further qualify in the area of up to date diagnostic methods.
 
Due to the currently very high proportion of female researchers at our institution we particularly encourage qualified males to apply for this position.
 
How to apply:
 
Applications should be sent in electronically as a single file containing the usual documents (letter of motivation, curriculum vitae, graduation documents, list of publications, a recent research article in English) and two reference addresses.
 
Contact details for applications:

Prof. Dr. Ludwig Niessen
Lehrstuhl für Technische Mikrobiologie
Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt
Technische Universität München
Weihenstephaner Steig 16
85350 Freising
Tel. 08161 715496
Fax 08161 713327
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