Where do different cells of hematopoietic lineage reside?

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sheriffabt
sheriffabt's picture
Where do different cells of hematopoietic lineage reside?

I am interested in counting cells that my project so I want to know where do different cells of lineage reside?

In order to count the cells, what are the ways I can do it?

I have access to FACS and also some markers... Any brilliant person please help!!!!!!!!!!!!!

marcus muench
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human, mouse or ????

human, mouse or ????

Embryo to Fetus to adult: Yolk sac, placenta, AGM/PAS, liver, spleen (small mammals only) and then bone marrow (marrow hematopoiesis can change sites as humans/animals grow). The thymus is another site mostly dedicated to T-cell development. When and if these sites are active depends on the species.

Mature cells circulate throughout the body but hematopoietic cells are enriched in the blood, spleen and lymph nodes. Different lymphoid tissues such as tonsils or mesenteric lymph nodes have different types of cells. There are also blood cells found in the peritoneum and the liver is also a major site for immune surveillance by different types of blood cells.

sheriffabt
sheriffabt's picture
its murine..

its murine..
I am basically trying to do some modelling
doing a winter proj in NCBS, and CAM , TIFR India
I have to count all cells of hematopoietic lineage
starting from HSC and get their no.
what strategy should I follow
hw shall I do that..