To apply: (link is external)https://aprecruit.berkeley.edu/apply/JPF01245(link is external)The Saijo lab in the Molecular Cell Biology Department at UC Berkeley seeks a Jr. Specialist. The goals of the Saijo lab are to understand the molecular mechanisms behind how genes and the environment cooperate to maintain homeostasis in the brain and how nuclear receptor-mediated epigenetic and transcriptional regulation can be used to restore normal brain function using mouse models of neuropsychiatric diseases. We are looking of a motivated Junior Specialists with and interest in tackling these questions using multidisciplinary approaches in combination of neuroscience, immunology, endocrinology and molecular biology.Responsibilities:
- Independent Research: running multiple mouse behavior assays to test the efficacy of compounds on different mouse models of neuropsychiatric disorders.
- Animal colony maintenance and genotyping: including, animal breeding, weaning, culling, and genotyping by tissue biopsy and assay by PCR with a standard thermocycler. Additionally, needs to be familiar with various techniques of mouse injection and surgery.
- Preparing next generation sequencing (NGS) libraries for RNA-seq, ChIP-seq, ATAC-seq and methylome analysis
- Basic tissue culture, transfection and analysis of phenotype of cell lines. Isolation of primary immune cells and brain cells from mice.
- DNA and RNA analysis: standard techniques required for molecular biology. Including DNA amplification and purification: including bacterial retransformation, amplification, purification by standard Qiagen kit, and quantification with a spectrophotometer. RNA isolation and cDNA synthesis and quantitative PCR set up and analysis.
- Lab management duties will include ordering, filling reagent requests, coordinating food supplies, and stock keeping. Supervising compliances with all safety and animal regulations, radiation safety.
Minimum/Basic Qualifications Required (By the time of application): Bachelor’s degree, or equivalent degree, in Molecular and Cell Biology, Neuroscience, Immunology or related. Additional Qualifications Required (by start date): The successful candidate will have at least 6 months of laboratory employment. Experience with tissue culture, cloning, genotyping, mouse handling and behavior assays Preferred Qualifications (by start date): Excellent communication skills and careful attention to detail. Appointment: This position reports to Dr. Kaoru Saijo. The initial appointment for 1 year with the expectation of extension based on satisfactory performance and availability of funding. The approximate start date of this position will be January 2017.Salary: $17.99/hour – $19.18/hour at 100% time, depending on qualifications. This position provides full benefits. To apply: https://aprecruit.berkeley.edu/apply/JPF01245 Interested individuals should submit application documents as PDFs, which includes, an updated curriculum vitae, and names with contact information for up to 2 individuals who have agreed to provide a reference for this specific position and a cover letter. (Letters of reference may be requested of the finalists). Specific questions regarding the recruitment can be directed to Sabastian Belser, HR Partner, firstname.lastname@example.org. This recruitment will remain open until filled. All letters will be treated as confidential per University of California policy and California state law. Please refer potential referees, including when letters are provided via a third party (i.e., dossier service or career center), to the UC Berkeley statement of confidentiality (http://apo.berkeley.edu/evalltr.html) prior to submitting their letters. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see: http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct. The department is interested in candidates who will contribute to diversity and equal opportunity in higher education through their work.