I want triple color staining using human blood ; CD3-FITC, CD19-PE, CD56-APC. For compensation, I also prepare single color stainings. I usually run the flow in FITC -> PE -> APC order. Somebody said PE -> FITC -> APC order is easy for compensation. Which order is better? What is approximate voltages for FS and SS? Is density plot better than dot plot for compensation? Does anyone know good web sites for flow cytometry compensation?