reconstitution of LPS from SIGMA

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lakshman
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reconstitution of LPS from SIGMA

I have purchased LPS from SIGMA, but no information regarding its reconstitution is available. Can anybody advise me on the solvent that is to be used, storage temperature etc.

Dbrfrsr
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We use both Sigma LPS and

We use both Sigma LPS and Ultra Pure LPS from List Biologicals, and for both we resuspend in water (to 1mg/mL) and heat to around 50 degrees Celcius with intermittent vortexing (or sonication).
It can be stored at 4 degrees short term, or in the freezer (-20 degrees is fine), indefinitely.
This information, including references, is from the information sheet that came with our List Biologicals LPS(listlabs.com).

vstolberg
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lakshman wrote:I have

lakshman wrote:

I have purchased LPS from SIGMA, but no information regarding its reconstitution is available. Can anybody advise me on the solvent that is to be used, storage temperature etc.

We use LPS from Sigma in our laboratory and reconstitute it in 1 or 2 mL of Dulbecco's PBS (also from Sigma), depending on the desired concentration, and store at 4C for up to a year (although we normally use the entire vial within one year). Hope that helps!

lakshman
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thank you so much for your

thank you so much for your input. its really appreaciated.

fallensparks
fallensparks's picture
 Hi, 

 Hi, 

I purchased LPS from Sigma, added sterile ddH2O to 1mg/ml, swirled gently and stored aliquots at -20. I am trying to use them now (6 months later) and find the LPS is completely ineffective even at very high concentrations.

Was my mistake to reconstitute in water and not PBS - and also not to heat/vortex/sonicate?

I notice also that pre-made solutions of LPS avaiable from vendors have a "Do Not Freeze" warning. However their data sheets say that LPS reconstituted in PBS can be frozen. 

I would be very grateful for any advice! 

Thanks!