PBMC Isolation: total starting volume of whole blood

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MetrixMel
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PBMC Isolation: total starting volume of whole blood

 I'm learning how to isolate PBMCs but do not know how much whole blood to start with if I need to have a final count of 10 million cells in each cryovial for storage.

I need about 200 vials to store as my control.

Does anyone know how many PBMCs are in 1ml of whole blood??

How many mL of whole blood do I need to isolate 2 billion PBMCs (10 million PBMCs in 200 cryovials).

g a
g a's picture
 Hi MetrixMel

 Hi MetrixMel

1 cubic mm contains about 4000-10000 WBC of which at max about 30% are PBMCs. Since 1ml =1000cu mm. You can derive accordingly.