Recommendations for suppliers of exoglycosidases wanted

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SWilliams
SWilliams's picture
Recommendations for suppliers of exoglycosidases wanted

Hey everybody,

I've just started carrying out some exoglycosidase digestions of N-glycans from recombinant glycoproteins. However, the B-galactosidase I bought doesn't seem to be very effective. Looking into other possible sources, the price seems to vary quite a lot, so rather than waste my money on another not particularly good enzyme, does anyone have any sources they can recommend?

Thanks

Chin Fen Teo
Chin Fen Teo's picture
Hi SWilliams,

Hi SWilliams,

Try either ProZyme or New England Biolabs. It usually takes SUPER long time to get decent cleavage (like 48 hours). Adding more enzyme after the first 24 hours incubation will help.

Good luck.