N-linked sugar question?

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glycoman
glycoman's picture
N-linked sugar question?

I am working on a plasma membrane protein that has multiple sites of N-glcosylation. It seems they are indispensible for function, ie cleavage with PNGase F destroys function. Can someone give me some basic information on what these structures do and how to determine what they are?

Thanks

Gman

R Bishop
R Bishop's picture
Here's a great basic

Here's a great basic explanation of all PTMs with emphasis on glycan-addition. Hope this helps

http://themedicalbiochemistrypage.org/protein-modifications.html

RB

Bryan Evans
Bryan Evans's picture
This is data shows the

This is data shows the separation of fetuin oligosaccharides on Unison UK-Amino:
http://www.imtakt.com/TecInfo/TI433E.pdf
Below is a link to the article that is referenced in the data:
http://glycob.oxfordjournals.org/cgi/content/abstract/8/7/685
 
 

ncwildb88
ncwildb88's picture
 http://www.nature.com/nrg

 http://www.nature.com/nrg/journal/v7/n7/abs/nrg1894.html

Sorry I uploaded the PDF from my computer. I thought it would be on the reply.