When I run my protein which has 3 consensus sites for N linked glycosylation on a IEF gel, I see multiple bands, very close to each other. What do these bands mean?
Also, when I run the lower molecular weight aggregates of the same protein the multiple bands decrease in pI in a regular manner. These could be glycosylation isoforms of the same protein. Can someone explain the shift in pI?
Thanks in advance,