how to know the cell H9C2 is fully differentiated? is there any marker gene

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LUNA
LUNA's picture
how to know the cell H9C2 is fully differentiated? is there any marker gene

i don't know whether this cell line H9C2 is suitable to do the next experient,  because i don't know whether H9C2 cell is fully differentiated, could anyone help me?  any test methods?

Thank you.

R Bishop
R Bishop's picture
Greetings LUNA,

Greetings LUNA,

Welcome to Scientist Solutions.  Any earlier thread is worth reading on these cells can be found here.

http://www.scientistsolutions.com/t1311-trouble+with+rat+heart+h9c2+cells.html

From what I know this cell line was derived from embryonic mouse tissue, so your question is a relevant one.

From www.atcc.org

Comments: H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.

Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation. Fusion occurs faster if the serum concentration in the medium is reduced to one percent.

I guess that would be one test method. 

Bishop

ayesha
ayesha's picture
sir

sir
hi
can u tell me how v convert liter in grams
regards