HA substrate gel zymography

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reshmareshma
reshmareshma's picture
HA substrate gel zymography

Hi....im a postgraduate student from a local university. Im currently doing a research on hyaluronidase, the enzyme that is implicated in osteoarthritis disease and my current project is to study the hyaluronidase activity in the chondrocyte culture using zymography method. I would like to ask help from anyone who have an idea on how to conduct this experiment and if possible, could anyone send me a copy of a reliable protocol on this method. I have tried finding journals on zymography and tried following the methods in the journal but i fail to get any results. I hope that someone can help me....thank you.