Deglycosylation of native proteins

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protdoc
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Deglycosylation of native proteins

Does anyone know of a good commerical kit for the deglycosylation of proteins under non-denaturing conditions?

homes2
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protdoc wrote:Does anyone

protdoc wrote:

Does anyone know of a good commerical kit for the deglycosylation of proteins under non-denaturing conditions?

Hi there,
Probably, there are some kit to deglycosylate glycoproteins, but I recommend high-concentrated PNGase-F. I have tried Prozyme enzyme (1-2 ul) to release N-glycans from native glycoproteins (10 ug) and it worked well. Good luck!

RoliWeis
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From my experience, the only

From my experience, the only one really working under non-denaturing conditions for moderate to highly glycosylated proteins is the EndoH kit from NEB!

rumym
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The question sounds ambiguous

The question sounds ambiguous. I suggest discriminating between enzymatic glycosylation and non-enzymatic glycosylation (glycation) of proteins. Means for deglycosylation in both cases are different.