I am working on in vitro expression analysis. I have transfected a mammalian cell line with a gene of wild type and mutant one. surprisingly, I found that the transcript level of the wild type gene was less than the mutant (which is against the logic theory where the wild type should be expressed more than the mutant)..... I wondered what is the reason behind the less expression of the wild type than the mutant one.... any idea because I got exhausted with this without finding an answer ????????