Transcription Factors

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Nagindra Prashad
Nagindra Prashad's picture
Transcription Factors

I would like to purify DNA binding protein that binds to a 50 BP oligo. This is a DIG-labelled oligo and DNA binding is identified by Gel Shift assays and by UV cross linking. I would like to purify this protein for sequencing and identification. I will appreciate any suggesstions for an easy mini purification methods.
Thank you.
 

rimanwan
rimanwan's picture
You can try the Qiagen Gel

You can try the Qiagen Gel Extraction Kit, it be told that can only work more than 70 bp, but actually, It can work with 40 or 50 bp.
BTW, don't believe Fermentas Gel Extraction Kit, it is trash really!